三重大学大学院医学系研究科生化学分野　竹本研究室

研究業績

原著論文

  1. Ozaki-Noma R, Wazawa T, Kakizuka T, Shidara H, Takemoto K, Nagai T "Positive-Type Reversibly Photoswitching Red Fluorescent Protein for Dual-Color Superresolution Imaging with Single Light Exposure for Off-Switching" ACS Nano, ASAP 2025 LINK
  2. Shidara H, Shirai T, Ozaki-Noma R, Jitsuki S, Nagai T, Takemoto K* "Optical inactivation of intracellular molecules by fast-maturating photosensitizing fluorescence protein, HyperNova" Commun. Biol. 7, 945, 2024 LINK
  3. Jahan A, Akter TM, Takemoto K, Oura T, Shitara A, Semba S, Nezu A, Suto S, Nagai T, Tanimura A "Insertion of circularly permuted cyan fluorescent protein into the ligand-binding domain of inositol 1,4,5-trisphosphate receptor for enhanced FRET upon binding of fluorescent ligand" Cell Calcium, 108, 102668, 2022
  4. Trusel M, Nuno-Perez A, Lecca S, Harada H, Lalive AL, Congiu M, Takemoto K, Takahashi T, Ferraguti F and Manuel M “Punishment-predictive cues guide avoidance through potentiation of hypothalamus-to-habenula synapses.“ Neuron 102(1):120-127, 2019 LINK
  5. Riani Y D, Matsuda T, Takemoto K, Nagai T. “Green monomeric photosensitizing fluorescent protein for photo-inducible protein inactivation and cell ablation.” BMC Biol. 16(1):50, 2018
  6. Takemoto K* (*co-corresponding), Iwanari H, Tada H, Suyama K, Sano A, Nagai T, Hamakubo T and Takahashi T* “Optical inactivation of synaptic AMPA receptors erases fear memory.” Nat. Biotechnol., 35(1):38-47, 2017 LINK
  7. Tada H, Miyazaki T, Takemoto K, Jitsuki S, Nakajima W, Koide M, Yamamoto N, Taguchi A, Kawai H, Komiya K, Suyama K, Abe H, Sano A and Takahashi T “Social isolation suppresses actin dynamics and synaptic plasticity through ADF/cofilin inactivation in the developing rat barrel cortex.” Sci. Rep. 7(1):8471, 2017
  8. Tada H, Miyazaki T, Takemoto K, Takase K, Jitsuki S, Nakajima W, Koide M, Yamamoto N, Komiya K, Suyama K, Sano A, Taguchi A and Takahashi T “Neonatal isolation augments social dominance by altering actin dynamics in the medial prefrontal cortex.” Proc Natl Acad Sci U S A., 113(45), E7097-E7105, 2016
  9. Sakamaki K, Ishii TM, Sakata T, Takemoto K, Takagi C, Takeuchi A, Morishita A, Takahashi H, Nozawa A, Shinoda H, Chiba K, Sugimoto H, Saito A, Tamate S, Satou Y, Jung SK, Matsuoka S, Koyamada K, Sawasaki T, Nagai T and Ueno N “Dysregulation of a potassium channel, THIK-1, targeted by caspase-8 accelerates cell shrinkage.” Biochem Biophys Acta., 1863(11):2766-2783, 2016
  10. Jitsuki S, Nakajima W, Takemoto K, Sano A, Tada H, Takahashi-Jitsuki A, Takahashi T, Nogo receptor signaling restricts adult neural plasticity by limiting synaptic AMPA receptor delivery., Cereb. Cortex, 26, 427-439, 2016
  11. Liu T, Yamaguchi Y, Shirasaki Y, ShikadaK, Yamagishi M, Hoshino K, Kaisho T, Takemoto K, Suzuki T, Kuranaga E, Ohara O and Miura M “Single-cell imaging of caspase-1 dynamics reveals all-or-none response of inflammasome signaling.” Cell Rep. 8(4):974-982, 2014
  12. Takemoto K, Matsuda T, Sakai N, Fu D, Noda M, Uchiyama S, Kotera I, Arai Y, Horiuchi M, Fukui K, Ayabe T, Inagaki F, Suzuki H and Nagai T. “SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation.” Sci.Rep. 3, 2629, 2013 LINK
  13. Ohtsuka K, Sato S, Sato Y, Sota K, Ohzawa S, Matsuda T, Takemoto K, Takamune N, Juskowiak B, Nagai T and Takenaka S　“Fluorescence imaging of potassium ions in living cells using a fluorescent probe based on a thrombin binding aptamer-peptide conjugate.” Chem Commun., 48, 4740–4742, 2012
  14. Yamaguchi Y, Shinotsuka N, Nonomura K, Takemoto K, Kuida K, Yoshida H and Miura M.　“Live imaging of apoptosis in a novel transgenic mouse highlights its role in neural tube closure.“ J.Cell.Biol. 195(6): 1047-1060, 2011
  15. Takemoto K, Matsuda T, McDougall M, Klaubert D, Hasegawa A, Los G, Wood K, Miyawaki A and Nagai T “Chromophore-assisted light inactivation of HaloTag fusion proteins labeled with eosin in living cells.” ACS Chem. Biol. 6(5):401-406, 2011 LINK
  16. Kuranaga E, Matsunuma T, Kanuka H, Takemoto K, Koto A, Kimura K and Miura M　“Apoptosis controls the speed of looping morphogenesis in Drosophila male terminalia.” Development 138(8):1493-1499, 2011
  17. Jitsuki S, Takemoto K, Kawasaki T, Tada H, Takahashi A, Becamel C, Sano A, Yuzaki M, Zukin RS, Ziff EB, Kessels HW and Takahashi T. “Serotonin mediates cross-modal reorganization of cortical circuits.” Neuron 69(4):780-792, 2011
  18. Takemoto K, Kuranaga E, Tonoki A, Nagai T, Miyawaki A and Miura M “Local initiation of caspase activation in Drosophila salivary gland programmed cell death in vivo.“ Proc Natl Acad Sci U S A. 104(33):13367-13372, 2007 LINK
  19. Kuranaga E, Kanuka H, Tonoki A, Takemoto K, Tomioka T, Kobayashi M, Hayashi S and Miura M “Drosophila IKK-Related Kinase regulates non-apoptotic function of caspases via degradation of IAPs.” Cell 126(3):583-596, 2006
  20. Kanuka H, Kuranaga E, Takemoto K, Hiratou T, Okano H and Miura M “Drosophila caspase transduces Shaggy/GSK-3b kinase activity in neural precuorsor development.” EMBO J. 24(21):3793-3806, 2005
  21. Takemoto K, Nagai T, Miyawaki A and Miura M “Spatio-temporal activation of caspase revealed by indicator that is insensitive to environmental effects.” J.Cell Biol. 160(2):235-243, 2003 LINK
  22. Uetsuki T, Takemoto K, Nishimura I, Okamoto M, Niinobe M, Momoi T, Miura M and Yoshikawa K “Activation of Neuronal Caspase-3 by Intracellular Accumulation of Wild-Type Alzheimer Amyloid Precursor Protein.” J.Neurosci. 19(16):6955-6964, 1999

総説・著書

  1. Shidara H, Jitsuki S and Takemoto K "Chromophore-assisted light inactivation of target proteins for singularity biology" Biophysics and Physicobiology, e211009, 2024 LINK
  2. 竹本研「光による分子不活性化操作とin vivoへの応用」日本薬理学会雑誌　157巻4号 2022
  3. Takemoto K "Optical manipulation of molecular function by chromophore-assisted light inactivation." Proc Jpn Acad Ser B Phys Biol Sci., 97(4):197-209, 2021 LINK
  4. 竹本研　「蛋白質機能の光操作とその応用」　横浜医学、70, 67-74, 2019
  5. Yamaguchi Y, Kuranaga E, Nakajima Y, Koto A, Takemoto K and Miura M. “In vivo monitoring of caspase activation using a fluorescence resonance energy transfer-based fluorescent probe. Regulated Cell Death.” Methods Enzymol. 544:299-325, 2014
  6. 竹本研、永井健治、宮脇敦史、三浦正幸　“アポトーシスの検出－生きた細胞・個体におけるカスパーゼ活性化のライブ検出”　蛍光・発光試薬の選び方と使い方　（羊土社）p143-146 , 2007
  7. 竹本研、三浦正幸 “カスパーゼの生理機能”　新世紀における蛋白質科学の進展―蛋白質核酸酵素増刊　46(11):1692-1696 , 2001
  8. 竹本研、三浦正幸 “細胞死シグナルの可視化-in vivoライブイメージングを目指して” 　実験医学　22(15):2136-2140, 2004
  9. 竹本研、三浦正幸 “進化的に保存された神経細胞死の実行機構”　放射線生物学研究  35(2): 143-151, 2000

知的財産権

  1. 特許名称：立体構造形成能が高い光増感タンパク質
    　発明者：竹本研
    　出願日：2022年6月27日
    　出願人：国立大学法人　三重大学、公立大学法人　横浜市立大学
    出願番号：特願2022-103027
    公開番号：特開    2024-003701
  2. 特許名称：AMPA型グルタミン酸受容体サブユニットを認識するモノクローナル抗体
    　発明者：竹本研・浜窪隆雄・岩成宏子・高橋琢哉
    　出願日：2014年7月29日
    　出願人：公立大学法人　横浜市立大学
    特許番号：特許第    6434736号
  3. 特許名称：光増感蛍光タンパク質
    発明者：永井健治・松田知己・高橋里佳・竹本研 出願日：2010年2月3日
    出願人：国立大学法人　北海道大学
    出願番号：特願2010-22603,  PCT/JP2011/052300
    公開番号：特開    2013-78264,  WO2011/096501 A1